The estimation of unsaturated fatty acids by reversed-phase partition chromatography

The separation of the C12-C18 fatty acids by reversed-phase partition chromatography.

The resolution of mixtures of C16-C24 normal-chain fatty acids by reversed-phase partition chromatography.

Stand oils (fish drying oils) made from the body oil of the pilchard (Sardina ocelktta Jenyns) are of commercial importance in South Africa and, as part of a general study of these and other polymerized oils (cf. Joubert & Sutton, 1952), the need arose for an analytical method suitable for the separation of mixtures of saturated and unsaturated acids having from 16 to 24 carbon atoms in the cha...

The estimation of fatty acids of intermediate chain length by partition chromatography.

In 1942 Smith (1) demonstrated that the silica gel partition chromatogram, first developed by Martin and Synge (2), would separate a mixture of formic, acetic, propionic, n-butyric, and n-valeric acids in chloroform-l per cent butanol into its component acids. In 1945 Ramsey and Patterson (3) confirmed this work and presented a partition chromatographic method for the qualitative separation and...

Biosynthesis of Unsaturated Fatty Acids by Bacilli

Significant relationships have been established between the initiation and rate of fatty acid A”-desaturase synthesis in Bacillus megaterium ATCC 14581 and controlled perturbations in culture temperature, cell growth, protein synthesis, and RNA synthesis. B. megaterium growing from inoculum at 35” contained neither unsaturated fatty acids nor the A5-desaturase responsible for their production. ...

Analysis of Fatty Acids in Mouse Cells Using Reversed-Phase High-Performance Liquid Chromatography

A reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed to analyze various fatty acids in recombinant mouse L cells. These fatty acids were the metabolites of oleic acid. A process was developed to extract fatty acids from the cell samples before RP-HPLC analysis. The samples were first saponified w~th 0.5 M NaOH in 96% ethanol then extracted with acidified ethyl ...